Full Project – THE PHYTOCHEMICAL CONSTITUENTS OF HEXANOLIC EXTRACT OF OCIMUM GRATISSIMUM (SCENT LEAVES)
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CHAPTER ONE
1.0 Introduction
1.1 Aim of Study
1.2 Literature Review
1.2.1 Phytochemical Screening
1.2.1.1 Flavonoid
1.2.1.2 Saponin
1.2.1.3 Tannin
1.2.1.4 Alkaloids
CHAPTER TWO
2.0 Materials and Methods
2.1 Materials Used
2.2 Reagents
2.3 Identification and Preparation of plant materials
2.4 Phytochemical analysis of hexanolic extract of O. gratissimum
2.4.1 Qualitative determination
2.4.1.1 Test for Alkaloids
2.4.1.2 Test for Flavonoids
2.4.1.3 Test for Saponin
2.4.1.4 Test for Phytosterol
2.4.1.5 Test for Phenol
2.4.1.6 Test for Steroids
2.4.1.1 Test for Tannins
2.4.2 Quantitative determination
2.4.2.1 Alkaloids determination
2.4.2.2 Saponin determination
2.4.2.3 Tannin determination
2.4.2.4 Flavonoid determination
2.4.2.5 Estimation of total Phenolic content
2.4.2.6 Reducing Power
CHAPTER THREE
3.0 Results
3.1 Alkaloid determination
3.2 Saponin determination
3.3 Tannin determination
CHAPTER FOUR
4.0 Discussion and Conclusion
4.1 Discussion
4.2 Conclusion
References
LIST OF TABLES
Table 1 – Showing the qualitative and phytochemical analysis of Ocimum grattissimum
Table 2 – Showing the tannic acid absorbance
Table 3 – Showing the quercetin absorbance
Table 4 – Showing the absorbance of reducing power
Table 5 – Showing the determination of total Phenolic content
Table 6 – Showing the phytochemical analysis of hexanolic extract of ocimum gratissimum
Table 7 – Bioactivity of compounds detected through GC-MS screening of hexane extract of scent leaves
LIST OF FIGURES
Figxure 1 – Graph showing the tannic acid standard curve
Figure 2 – Graph showing the quercetin standard curve
Figure 3 – Graph showing the determination of reducing power
Figure 4 – Graph showing the determination of total phenolic content
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