Full Project – DISTRIBUTION OF THIAMINASE IN DIFFERENT TISSUES OF SOME FISH SPECIES
Click here to Get this Complete Project Chapter 1-5
ABSTRACT
Thiaminase degrade thiamine into separate thiazole and pyrimidine moieties, an enzyme that cleaves vitamin B1 and are sporadically distributed among prokaryotes and eukaryotes. The excess thiaminase in fish tissues causes thiamine deficiency syndrome like neurological and cardiac disorder, it also causes reproductive disorder in fishes and finally leads to death. Thiaminase in human causes thiamine deficiency which leads to beriberi and Wernicke Korsakov syndrome or Wernicke paralysis, it also prevent the conversion of carbohydrate to glucose. Fishes rich in thiaminase are not good for human consumption because it leads to chronic thiamine deficiency.
This present study was carried out to determine the distribution, level and the effect of thiaminase in different tissues of some fish species. A total number of 21 tissues were extracted from 7 different fish species (Gills, gut and flesh i.e. 3 from each). In the tissue, Tilapia Zilli gut had the highest thiaminase activity (27.05±2.08) while Parachanna Africana gill had the lowest activity (0.09±0.12). Tilapia Zilli gut also have the highest specific activity (0.0058±0.0004) Parachanna Africana gill has the lowest specific activity (0.0000±0.0000). Clarias Gariepinus flesh has the highest protein concentration (14387.44±179.13) Hepsetus Odoe gut has the lowest protein concentration (791.28±13.33). Saroterodon Galilaeus species has the highest level of thiaminase activity (10.136ª) and Parachanna Obscura species has the lowest level of thiaminase activity (0.463ᵇ). Tilapia Zilli species has the highest specific activity of (0.0023ª) while Parachanna Africanna species has the lowest specific activity of (0.0002ᶜ).
Gut has the highest thiaminase activity of (11.315ª) while flesh has the lowest thiaminase activity of (1.916ᵇ). Gut also has the highest specific activity of (0.00304ª) and flesh also has the lowest specific activity of (0.00022ᵇ). Therefore, the significance of the study is that excess thiaminase degrades thiamin and causes thiamin deficiency in fishes which leads to various ailments in fish and finally leads to death.
TABLE OF CONTENTS
CHAPTER ONE
1.1 INTRODUCTION
1.2 LITERATURE REVIEW
1.2.1 HISTORY OF THIAMINASE
1.2.2 CLASSIFICATION
1.2.3 DISTRIBUTION AND OCCURRENCE
1.2.4 THIAMINASE CATALYSIS
1.2.5 THIAMINASE 1
1.2.6 THIAMINASE 11
1.3 FISH TISSUES
1.3.1 FISH GILLS
1.3.2 FISH FLESH
1.3.3 FISH GUT
1.4 FISHES
1.5 FISH SPECIES
1.5.1 TILAPIA ZILLI
1.5.2 HEPSETUS ODOE
1.5.3 PARACHANNA OBSCURA
1.5.4 CLARIAS GARIEPINUS
1.5.5 HETEROTIS NILOTICUS
1.5.6 PARACHANNA AFRICANA
1.5.7 SAROTERODON GALILAEUS
CHAPTER TWO
2.1 MATERIALS
2.1.1 CHEMICALS AND REAGENTS
2.1.2 EQUIPMENTS
2.2 METHODS
2.2.1 PREPARATION OF BUFFER (PHOSPHATE BUFFE
2.2.2 HOMOGENIZATION OF SAMPLES (GILLS, GUT AND
FLESH)
2.2.3 FILTRATION USING CHEESE CLOTH
2.2.4 CENTRIFUGATION OF THE SUPERNATANT EXTRACT
2.2.5 PROTEIN DETERMINATION
2.2.6 THIAMINASE ASSAY
CHAPTER THREE
3.1 RESULTS
3.1.1 THIAMINASE ACTIVITY TABLE
CHAPTER FOUR
4.1 DISCUSSION CONCLUSION AND RECOMMENDATION
4.1.1 DISCUSSION
4.1.2 CONCLUSION
4.1.3 RECOMMENDATION
REFERENCES.
Get the Complete Project
This is a premium project material and the complete research project plus questionnaires and references can be gotten at an affordable rate of N3,000 for Nigerian clients and $8 for International clients.
Click here to Get this Complete Project Chapter 1-5
You can also check other Research Project here:
- Accounting Research Project
- Adult Education
- Agricultural Science
- Banking & Finance
- Biblical Theology & CRS
- Biblical Theology and CRS
- Biology Education
- Business Administration
- Computer Engineering Project
- Computer Science 2
- Criminology Research Project
- Early Childhood Education
- Economic Education
- Education Research Project
- Educational Administration and Planning Research Project
- English
- English Education
- Entrepreneurship
- Environmental Sciences Research Project
- Guidance and Counselling Research Project
- History Education
- Human Kinetics and Health Education
- Management
- Maritime and Transportation
- Marketing
- Marketing Research Project 2
- Mass Communication
- Mathematics Education
- Medical Biochemistry Project
- Organizational Behaviour
- Political Science
- Psychology
- Public Administration
- Public Health Research Project
- More Research Project
- Transportation Management
- Nursing
Full Project – DISTRIBUTION OF THIAMINASE IN DIFFERENT TISSUES OF SOME FISH SPECIES
Frequently Asked Questions
Our Customers are Happy
Ademola A.
I was skeptical at first, but after placing my order, my full project arrived in my email in under 15 minutes! The process was smooth, clear, and professional. Truly amazing service!
Kwabena K.
I needed a custom project on a new topic. researchprojecttopics.com.ng delivered within 3 days, and the quality was outstanding. They even guided me on how to defend it. Highly recommend!
Michael H.
Fast, reliable, and very professional. My research project was delivered on time, with no hidden charges. The team is trustworthy and supportive.
Fatou B.
I got my full project in minutes and my custom request within 3 days. Their communication is clear, and the material is top-notch. Excellent experience!
James O.
https://researchprojecttopics.com.ng is a lifesaver! My project was delivered exactly as requested. The team is friendly, professional, and highly responsive. Very satisfied!
Ngozi E.
I was worried about paying online, but the team reassured me and delivered my complete project instantly. Transparent and professional service!
Ama S.
I requested a custom topic project and received it in just 3 days. The guidance and quality were excellent. I recommend Https://researchprojecttopics.com.ng to everyone!
Sarah W.
The service is dependable and efficient. My project arrived on time, and every step was transparent. Truly a professional service I trust.
Emmanuel T.
Fast and reliable. My full project was delivered in minutes, and the custom project in 3 days. Communication was excellent throughout.
Aisha N.
Extremely satisfied with the service. My project was delivered promptly, fully transparent, and of high quality. A trustworthy academic partner!